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c/coa·posted 1 year ago by u/milos_trevino

three years of certificate of analysis threads, summarised so you do not have to read them

COA Receipts ×4 Well Actually ×2 Long Haul ×2

Right, the purity question again, but with numbers this time.

I have 24 data points over 10 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign.

The thing I would flag for anyone new: the effect I am describing showed up at week 90, not week 44. People give up long before the interesting part.

Interested in whether this matches other people's logs or whether I am an outlier.

5,100 up / 703 down88% upvoted70 commentsid 10suw917 Apr 2025

70 comments

30 in this archive, depth 5

best — the order this archive was captured in

u/wanjiru_varga-27 points·1 year ago

Did they give you the chromatogram or just the summary line?

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[deleted]1 point·1 year ago

[deleted]

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u/freya_baptista1 point·1 year ago

the counterion is part of the mass you paid for

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u/milos_vanhecke355 points·1 year ago

PDF metadata is checkable in fifteen seconds

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u/milos_trevinoOP227 points·1 year ago

PDF metadata is checkable in fifteen seconds

Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.

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u/milos_trevinoOP160 points·1 year ago

area% is not mass%, and here is exactly why it matters

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u/milos_trevinoOP203 points·1 year ago

The COA I was holding said 96.8% area and I thought it was trash. Turns out the method was solid and the impurity profile was clean.

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u/nikhil_lindqvist127 points·1 year ago

Strongly agree. Mass% and area% are answering different questions on the same document.

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u/dario_vermeulen231 points·1 year ago

Water content on the document? That affects your concentration math.

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u/teodor_lokken193 points·1 year ago

axis labels included, always

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u/bastian_aalto94 points·1 year ago

Are you looking at area% or net peptide content? Different numbers.

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u/soren_nkemelu155 points·1 year ago

the method line is doing real work, not just window dressing

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u/certified_referenceQC57 points·1 year ago

This is the correct framing. The COA structure matters as much as the number.

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u/purity_pedantMOD64 points·1 year ago

Tracking number removed. It identifies both ends of a shipment.

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u/tuva_aalto16 points·1 year ago

PDF metadata is checkable in fifteen seconds

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u/tove_ogunleye7 points·1 year ago

area% is not mass%, and here is exactly why it matters

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u/neha_erdogan94 points·1 year ago

What is the method line say? Not the technique, the actual method.

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u/tidy_vialdrawer_watch2825 points·1 year ago

chromatogram or it did not happen

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u/halima_boateng15 points·1 year ago

Batch number and date of analysis? Both are load-bearing.

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u/teodor_lokken21 points·1 year ago

area% is not mass%, and here is exactly why it matters

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u/emil_agyeman74 points·1 year ago

Did they give you the chromatogram or just the summary line?

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u/anders_kuusela35 points·1 year ago

area% is not mass%, and here is exactly why it matters

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u/liv_nkemelu24 points·1 year ago

area% is not mass%, and here is exactly why it matters

Yes, exactly this, and it is the bit that took me 53 weeks to accept.

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u/blunt_coldbox2919 points·1 year ago

purity and net content are two different numbers, same vial

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u/hugo_bergstrom0 points·1 year ago

no "is this good?" without saying what the compound and claimed purity are

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u/wrong_network_w35 points·1 year ago

area% is not mass%, and here is exactly why it matters

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u/step_therapy_s28 points·1 year ago·edited

Batch number and date of analysis? Both are load-bearing.

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u/kavya_verhoeven35 points·1 year ago

chromatogram or it did not happen

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u/liv_nkemelu22 points·1 year ago

Not to be pedantic but certificate of analysis and COA are being used interchangeably and they are not interchangeable in real life.

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u/camila_lindqvist39 points·1 year ago

post the whole document, not the headline number

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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