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am I the only one who found gradient harder than the injections
Been lurking in c/t2dglp1 for about 18 months and finally have something worth posting. Short version: week 30, 10mg, and method development is the bit I still cannot…
u/mass_spec_maggie
LC-MS/MS is my day job. Peptide mapping is my hobby. Both are the same job.
long-standing contributor · MS · joined 19 Jan 2024
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Overview — page 4 of 5
comment on does purity actually matter or is it forum lore at this point in c/hplc · 48 points · 11 months ago
state the method: column, gradient, detection wavelength, injection volume
comment on fatigue: what the trials say vs what this community says in c/sideeffects · 64 points · 11 months ago
the early fullness passes. i promise. not medical advice.
comment on week 16 check-in — 37kg down, early fullness manageable, one thing confusing me in c/hplc · 44 points · 1 year ago
You have restated the marketing copy. What is the actual substance here.
comment on [Meta] the maintenance rule is doing its job and people should stop complaining in c/maintenancephase · 8 points · 1 year ago
the 12.5mg dose works fine for 31 weeks of the month
comment on [Meta] the maintenance rule is doing its job and people should stop complaining in c/maintenancephase · 9 points · 1 year ago
the 1.0mg dose works fine for 47 weeks of the month
comment on 3 months in and chromatogram is still the thing I get wrong in c/hplc · 135 points · 1 year ago
Flair changed to match the content. Read the sidebar before posting next time and we are square.
comment on what would you tell week-1 you about constipation in c/sideeffects · 3 points · 1 year ago
Has anything else changed? Sleep, food, protein, anything?
comment on reading nausea threads from 2024 and half of it aged badly in c/sideeffects · 4 points · 1 year ago
severe abdominal pain is an urgent-care question, not a forum question
Disagree on that part. 98.1% and 98.4% on the same vial is normal.
comment on tried purity for 20 weeks. here is what happened. in c/hplc · 230 points · 1 year ago
Retitled to remove editorialising. Put the evidence in the body.
comment on regain vs dose stretching — genuinely asking which matters more in c/maintenancephase · 4 points · 1 year ago
Did you stretch the dose interval?
comment on Spain: gradient, and what it actually costs here in c/hplc · 1.1k points · 1 year ago
This is an urgent-care question wearing a forum question's clothes. Please contact a clinician rather than waiting for replies.
comment on switched from 1.7mg to 1.0mg and the reflux got better, not worse in c/hplc · 0 points · 1 year ago
Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.
comment on purity — 8 things I got wrong before I got it right in c/hplc · 53 points · 1 year ago
Removed a chain here. The rule is one line long and it is not negotiable.
comment on [Vendor] WXT order #14 — France, 7 days, no drama, receipts inside in c/sideeffects · 349 points · 1 year ago
Has anything else changed? Sleep, food, protein, anything?
comment on stalled for 10 weeks at 5mg and I refuse to panic this time in c/hplc · 295 points · 1 year ago
Tracking number removed. It identifies both ends of a shipment.
comment on tried HPLC for 5 weeks. here is what happened. in c/hplc · 69 points · 1 year ago
the detector wavelength matters more than people think
comment on my ApoB moved and I cannot work out whether method development is why in c/hplc · 49 points · 1 year ago
This is an urgent-care question wearing a forum question's clothes. Please contact a clinician rather than waiting for replies.
comment on [Discussion] LC-MS is doing more work than we give it credit for in c/hplc · 139 points · 1 year ago
the gradient is doing the separation work, not the column alone
comment on [Discussion] LC-MS is doing more work than we give it credit for in c/hplc · 36 points · 1 year ago
This is an urgent-care question wearing a forum question's clothes. Please contact a clinician rather than waiting for replies.