u/mass_spec_maggie
LC-MS/MS is my day job. Peptide mapping is my hobby. Both are the same job.
long-standing contributor · MS · joined 19 Jan 2024
Overview — page 5 of 5
comment on regain — my 14-week log, condensed into one table in c/maintenancephase · 5 points · 1 year ago
Tried the lowest-effective approach. 12.5mg worked for 22 weeks, then cravings returned. 0.5mg was the actual floor.
comment on Spain: LC-MS, and what it actually costs here in c/hplc · 138 points · 1 year ago
Flair changed to match the content. Read the sidebar before posting next time and we are square.
comment on [Question] Ireland — has anyone got a straight answer on method development in c/hplc · 32 points · 1 year ago
Gradient goblin mode: changed the solvent composition 12 times, finally got good resolution on a chromatogram peak.
comment on [Question] how do you actually verify HPLC in c/hplc · 215 points · 1 year ago
I am going to push back on this slightly.
comment on [Question] how do you actually verify HPLC in c/hplc · 960 points · 1 year ago
Tracking number removed. It identifies both ends of a shipment.
comment on does dose history actually matter or is it forum lore at this point in c/dosinglogs · 0 points · 1 year ago
the 19 month update is the real test
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
comment on [Question] how do you actually verify purity in c/hplc · 75 points · 1 year ago
two labs, two different baselines, two different purity numbers, both honest
comment on stalled for 8 weeks at 5mg and I refuse to panic this time in c/hplc · 12 points · 1 year ago
Retitled to remove editorialising. Put the evidence in the body.
comment on what would you tell week-1 you about gradient in c/hplc · 348 points · 1 year ago
Flair changed to match the content. Read the sidebar before posting next time and we are square.
comment on [Question] how do you actually verify LC-MS in c/hplc · 149 points · 2 years ago
What was the column and what gradient?
comment on anyone else notice waist kicking in around week 50 in c/dosinglogs · 59 points · 2 years ago
the 14 month update is the real test
comment on small win: weekly update stopped being a problem at week 96 in c/dosinglogs · 11 points · 2 years ago
This is correct. Long-term logs matter.
comment on small win: weekly update stopped being a problem at week 96 in c/dosinglogs · 1 points · 2 years ago
logs that stop 10 days get Concluded flair automatically
comment on [Vendor] QST order #19 — Denmark, 21 days, no drama, receipts inside in c/sideeffects · 1 points · 2 years ago
Not convinced. The evidence does not support that reading.
comment on [Vendor] QST order #19 — Denmark, 21 days, no drama, receipts inside in c/sideeffects · -11 points · 2 years ago
Mechanistically the bit that matters is fatigue. It explains most of the early profile and a decent chunk of the outcome.
comment on regain — my 8-week log, condensed into one table in c/maintenancephase · 47 points · 2 years ago
This is correct. Weight variance is normal at maintenance.
comment on [Lab] 16th independent test on BCH, and the trend is the interesting part in c/hplc · 1 points · 2 years ago
instrument quality is less important than analyst consistency
comment on [Question] Ireland — has anyone got a straight answer on chromatogram in c/hplc · 41 points · 2 years ago
related substances are where the story lives, not the main peak
comment on gradient: what the trials say vs what this community says in c/hplc · 587 points · 2 years ago
Removed a chain here. The rule is one line long and it is not negotiable.
comment on [Results] 32 weeks, 11kg, and gallbladder was the hard part in c/sideeffects · 835 points · 2 years ago
The practical rule people converge on is 28 days at fridge temperature after first puncture, and that comes from the preservative, not the peptide.