method development — 10 things I got wrong before I got it right
Been lurking in c/progresspics for about 6 months and finally have something worth posting.
Short version: week 50, 15mg, and integration is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 15 threads, and the answers split roughly down the middle.
What I actually want to know is whether the people saying it does not matter have measured it, or whether it is just repeated confidently. Happy to be told I am overthinking this — I probably am — but I would rather overthink it now than at week 44.
best — the order this archive was captured in
Not convinced. The evidence does not support that reading.
Half-life is about 168 hours, so steady state lands around week 2–5. Practical consequence: what you feel in week 1 is not what that dose does.
This is the correct method framing. Column and gradient are load-bearing fields.
Yeah, the chromatogram profile matters more than the headline number.
Not to be pedantic but purity and HPLC are being used interchangeably and they are not interchangeable in real life.
Did they post the raw trace or just the summary?
The PeptideMeter result being close to my own HPLC is the signal, not the absolute number. Both are right.
Injection volume and flow rate? Those affect resolution.
instrument quality is less important than analyst consistency
Yeah, the chromatogram profile matters more than the headline number.
Rodent data is rodent data. Dose scaling is not linear and the models tell you what to investigate, not what to expect.
Flair changed to match the content. Read the sidebar before posting next time and we are square.
I am going to push back on this slightly.
This is the "correlation is mechanism" thing again. You changed three variables at once.
a round-robin on baseline integration would be genuinely useful
Rodent data is rodent data. Dose scaling is not linear and the models tell you what to investigate, not what to expect.
- 1Rodent data is rodent data. Dose scaling is not linear and the models tell…5 comments in this branch · started by u/rosa_sandvik