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c/hplc·submitted 1 year ago by u/marit_mwangi

how much of what we believe about gradient actually comes from purity threads

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Genuine question, and the title is the question: how much of what we believe about gradient actually comes from purity threads. UV response is not uniform across species. At 214nm you are looking at the peptide bond, which is why it is the working wavelength for this class; at 280nm you only see tryptophan, tyrosine…

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6 comments, started 1 year ago
u/endotoxin_elliemicro626 points·1 year ago

Mass spectrometry answers identity. UV purity answers relative quantity under the run conditions. A document with one and not the other is answering half the question, and the half it answers should be stated.

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u/egfr_watcher162 points·1 year ago

axis labels or the trace is decoration

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u/marit_mwangiOP93 points·1 year ago·edited

What wavelength, and what was the gradient?

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