GLPHubglpresearchhub.com
Read-only archive. GLP Research Hub is a static community record — nothing here is for sale, no account is needed, and no vote you cast is counted. Why?
Single comment threadYou are looking at one branch of [Question] how do you actually verify LC-MS — 41 comments in the full submission. View in context.
8.1k
c/hplc·submitted 2 years ago by u/sofia_petrescu

[Question] how do you actually verify LC-MS

Questionbranch of 7 comments

Right, the integration question again, but with numbers this time. I have 13 data points over 6 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I am…

Read the full submission and all 41 comments →

This branch

7 comments, started 2 years ago
u/freya_baptista419 points·2 years ago

area% and mass% are not the same and this whole post conflates them

replysharereportpermalink
u/erez_yilmaz122 points·2 years ago·edited

Not convinced. The evidence does not support that reading.

replysharereportpermalink
u/first_hundred66 points·2 years ago

No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.

replysharereportpermalink
[removed]307 points·2 years ago

[removed by moderator]

replysharereportpermalink
u/careful_gradient75 points·2 years ago

chromatogram or it did not happen

replysharereportpermalink
u/rina_bergstrom109 points·2 years ago

That is net peptide content, not purity. Different number, different meaning.

replysharereportpermalink
u/blunt_coldbox_2024224 points·2 years ago

Strongly agree. Largest single impurity tells a different story than total purity.

replysharereportpermalink

← back to the whole thread

About c/hplc

The analytical deep end: RP-HPLC methods, gradient design, integration decisions, LC-MS confirmation, amino-acid analysis, water content, and why two labs can report 98.1% and 96.4% on the same vial and both be right. Post the chromatogram, not the summary line.

32kmembers
130submissions
Sep 2023created
submissions / month, last year
Sponsored

Sigma-Aldrich Standards

Certified reference materials for peptide identity and purity work.

sigmaaldrich.com
More in c/hplc
c/hplc rules
  1. Chromatogram or it did not happen. Axis labels included.
  2. State the method: column, gradient, detection wavelength, injection volume.
  3. Area% is not mass%. Posts that conflate them get a correction flair, not a removal.
  4. Independent community. Nobody here sells anything, and anyone who tries is banned.
  5. Not medical advice. Describe what you did; never prescribe to a stranger.
  6. Claims need evidence. Batch numbers, dated screenshots, independent test reports, or a citation.
  7. No referral links, discount codes or affiliate URLs. Permanent ban, no appeal.
  8. No contact handles, wallet addresses or tracking numbers — they identify people.
  9. Be recognisably decent. Disagree hard, insult nobody.
Full rules and how they are enforced →
Moderators
u/sig_figs_samu/hplc_hobbyistu/peak_area_peteu/mass_spec_maggie
Volunteers. Unpaid, unaffiliated, and reachable through modmail only.
Before you read on

Several compounds discussed on GLP Research Hub are sold for research use only and are not approved for human use anywhere. Nothing here is medical advice and none of it is written by your clinician. If a post reads like an instruction, treat it as a description of what one stranger did.

Full disclaimer →