does purity actually matter or is it forum lore at this point
chromatogram. That is the whole post, but I will justify it. Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, muscle cramps, the endless dose arguments — most of it resolves if you sort chromatogram out first, and almost nobody does. I say this having got it wrong for 10…
This is the correct method framing. Column and gradient are load-bearing fields.
chromatogram or it did not happen
Yeah, the chromatogram profile matters more than the headline number.
co-elution is real and LC-MS is usually not enough to resolve it
Not to be pedantic but gradient and integration are being used interchangeably and they are not interchangeable in real life.
Not to be pedantic but gradient and integration are being used interchangeably and they are not interchangeable in real life.
Adding to this: method development is doing more work than the comment implies.
Not to be pedantic but gradient and integration are being used interchangeably and they are not interchangeable in real life.
Yes, exactly this, and it is the bit that took me 34 weeks to accept.