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c/hplc·submitted 2 years ago by u/saskia_bakker

[Results] 52 weeks, 35kg, and integration was the hard part

Correctionbranch of 9 comments

I have had 21 orders now and I keep a table, so here is the honest summary rather than a vibe. Material has been consistent. Communication has not. Shipping has varied by about 12 days on nominally the same lane, which matters more in summer than in February. The independent number came back 93.7% against a claimed…

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9 comments, started 2 years ago
u/fatima_yildiz134 points·2 years ago

co-elution is real and purity is usually not enough to resolve it

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[deleted]55 points·2 years ago

[deleted]

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u/marit_mwangi42 points·2 years ago

integration decision on a shoulder changes your number by half a point on its own

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u/mateusz_mensah19 points·2 years ago

Strongly agree. Largest single impurity tells a different story than total purity.

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The analytical deep end: RP-HPLC methods, gradient design, integration decisions, LC-MS confirmation, amino-acid analysis, water content, and why two labs can report 98.1% and 96.4% on the same vial and both be right. Post the chromatogram, not the summary line.

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