tried method development for 12 weeks. here is what happened.
Right, the method development question again, but with numbers this time. I have 15 data points over 32 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I…
Water content matters more than people think. If a vial is carrying residual moisture, your "10mg" is not 10mg of peptide.
Half-life is about 168 hours, so steady state lands around week 6–5. Practical consequence: what you feel in week 1 is not what that dose does.
axis labels included, always
Strongly agree. Largest single impurity tells a different story than total purity.
related substances are where the story lives, not the main peak
Half-life is about 168 hours, so steady state lands around week 6–5.
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.
Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.
A shoulder on the main peak that 6 analysts integrated 15 different ways. Same trace, 96.2 spread.
area% and mass% are not the same and this whole post conflates them
A shoulder on the main peak that 6 analysts integrated 15 different ways.
Yes, exactly this, and it is the bit that took me 14 weeks to accept.
Strongly agree. Largest single impurity tells a different story than total purity.