three years of HPLC threads, summarised so you do not have to read them
chromatogram. That is the whole post, but I will justify it. Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, reflux, the endless dose arguments — most of it resolves if you sort chromatogram out first, and almost nobody does. I say this having got it wrong for 24 months. My…
a round-robin on baseline integration would be genuinely useful
related substances are where the story lives, not the main peak
Yeah, the chromatogram profile matters more than the headline number.
Respectfully this is a sample of one presented as a finding.
co-elution is real and chromatogram is usually not enough to resolve it
related substances are where the story lives, not the main peak
Disagree on that part. 96.8% and 96.2% on the same vial is normal.
Not to be pedantic but chromatogram and method development are being used interchangeably and they are not interchangeable in real life.
The PeptideMeter result being close to my own HPLC is the signal, not the absolute number. Both are right.
the detector wavelength matters more than people think