[Question] how do you actually verify HPLC
Something I noticed reading old threads that I have not seen said out loud. The advice on integration in this community changed substantially around the start of last year, and nobody went back and updated the older posts. So depending on which thread the search engine hands you, you get two contradictory answers…
the detector wavelength matters more than people think
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Yeah, the chromatogram profile matters more than the headline number.
the detector wavelength matters more than people think
area% and mass% are not the same and this whole post conflates them
Not to be pedantic but chromatogram and method development are being used interchangeably and they are not interchangeable in real life.
related substances are where the story lives, not the main peak