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c/hplc·submitted 10 months ago by u/mass_spec_maggie

genuine question about gradient that I am slightly embarrassed to ask

Questionbranch of 10 comments

Been lurking in c/t2dglp1 for about 13 months and finally have something worth posting. Short version: week 34, 15mg, and gradient is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 11 threads, and the answers split roughly down the middle. What I actually want to know is…

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10 comments, started 10 months ago
u/nabila_espinoza494 points·10 months ago·edited

No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.

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u/niels_lindqvist135 points·10 months ago

instrument quality is less important than analyst consistency

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u/hplc_hobbyistruns their own column93 points·10 months ago

Disagree but this is the good kind of wrong — it is specific enough to be checked.

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u/vikram_asante41 points·10 months ago

Detection wavelength? purity and that changes at different wavelengths.

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u/micro_bump_mick-31 points·10 months ago

instrument quality is less important than analyst consistency

Yes, exactly this, and it is the bit that took me 28 weeks to accept.

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u/matias_salgado1 point·10 months ago

state the method: column, gradient, detection wavelength, injection volume

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u/first_hundred1 point·10 months ago

the detector wavelength matters more than people think

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[removed]354 points·10 months ago

[removed by moderator]

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