LC-MS: the version I wish someone had shown me in week 1
Something I noticed reading old threads that I have not seen said out loud. The advice on LC-MS in this community changed substantially around the start of last year, and nobody went back and updated the older posts. So depending on which thread the search engine hands you, you get two contradictory answers with the…
state the method: column, gradient, detection wavelength, injection volume
This is the correct method framing. Column and gradient are load-bearing fields.
What was the column and what gradient?
instrument quality is less important than analyst consistency
This is the correct method framing. Column and gradient are load-bearing fields.
area% and mass% are not the same and this whole post conflates them
the detector wavelength matters more than people think
co-elution is real and integration is usually not enough to resolve it
area% and mass% are not the same and this whole post conflates them
Yes, exactly this, and it is the bit that took me 90 weeks to accept.
Detection wavelength? method development and that changes at different wavelengths.
axis labels included, always
axis labels included, always
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.