someone explain LC-MS to me like I have not read a paper in years
someone explain LC-MS to me like I have not read a paper in years. Searched first, found three threads that contradict each other, hence the post. Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of peak width and…
What did system suitability look like on that sequence?
Same method at both labs, or two different gradients?
Chased a ghost peak for three weeks. It was the plumbing. It is always the plumbing.
Do you have the mass, or only the UV trace?
Is that baseline drawn by the software or by hand?
214nm sees the peptide bond, 280nm sees the aromatics
Same method at both labs, or two different gradients?
This is the whole methodological point of the board in one line.