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c/hplc·submitted 5 months ago by u/hydration_hank

the LC-MS question that gets asked weekly, answered properly

Questionbranch of 5 comments

Been lurking in c/t2dglp1 for about 6 months and finally have something worth posting. Short version: week 18, 2.5mg, and gradient is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 33 threads, and the answers split roughly down the middle. What I actually want to know is…

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5 comments, started 5 months ago
u/isabela_nilsen0 points·5 months ago

Injection volume and flow rate? Those affect resolution.

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u/hydration_hankOP1 point·5 months ago

instrument quality is less important than analyst consistency

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u/first_hundred1 point·5 months ago

Not to be pedantic but method development and integration are being used interchangeably and they are not interchangeable in real life.

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u/hydration_hankOP1 point·5 months ago

What was the column and what gradient?

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u/hydration_hankOP1 point·5 months ago

This is the "correlation is mechanism" thing again. You changed three variables at once.

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