am I the only one who found integration harder than the injections
Long-ish post, sorry. tl;dr at the bottom. I have been tracking LC-MS against ApoB for 17 weeks because I could not find anyone who had. The correlation is weaker than I expected, which is itself mildly interesting given how confidently people link the two in here. Caveats up front: one person, one lab, one assay, no…
Retitled to remove editorialising. Put the evidence in the body.
A shoulder on the main peak that 2 analysts integrated 23 different ways. Same trace, 97.6 spread.
Yeah, the chromatogram profile matters more than the headline number.
a round-robin on baseline integration would be genuinely useful
related substances are where the story lives, not the main peak
Strongly agree. Largest single impurity tells a different story than total purity.
the gradient is doing the separation work, not the column alone
I am going to push back on this slightly.
instrument quality is less important than analyst consistency
the detector wavelength matters more than people think
the gradient is doing the separation work, not the column alone
Ran the same vial on two columns, different purity, both right, different separation. Method matters more than equipment.
state the method: column, gradient, detection wavelength, injection volume
area% and mass% are not the same and this whole post conflates them