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c/hplc·submitted 7 months ago by u/lina_novak

the integration question that gets asked weekly, answered properly

Correctionbranch of 7 comments

Genuinely asking, not being difficult. Everyone in c/glp1eu repeats that method development is important. I believe it, but I have never seen anyone show why, and when I search I get 23 threads of people agreeing with each other. Is there a paper? Is there a measurement? Or is this one of those things that is true…

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7 comments, started 7 months ago
u/ignacio_vanhecke10 points·7 months ago

Yeah, the chromatogram profile matters more than the headline number.

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u/rina_bergstrom6 points·7 months ago

Did they post the raw trace or just the summary?

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u/bilal_adebayo3 points·7 months ago

co-elution is real and gradient is usually not enough to resolve it

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u/lina_novakOP-12 points·7 months ago

state the method: column, gradient, detection wavelength, injection volume

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u/careful_gradient8 points·7 months ago

axis labels included, always

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u/vikram_asante5 points·7 months ago

axis labels included, always

Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.

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u/lina_novakOP5 points·7 months ago

state the method: column, gradient, detection wavelength, injection volume

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