my A1c moved and I cannot work out whether method development is why
Right, the LC-MS question again, but with numbers this time.
I have 2 data points over 16 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign.
The thing I would flag for anyone new: the effect I am describing showed up at week 20, not week 69. People give up long before the interesting part.
Interested in whether this matches other people's logs or whether I am an outlier.
best — the order this archive was captured in
Yeah, the chromatogram profile matters more than the headline number.
Did they post the raw trace or just the summary?
a round-robin on baseline integration would be genuinely useful
A shoulder on the main peak that 20 analysts integrated 9 different ways. Same trace, 98.0 spread.
co-elution is real and integration is usually not enough to resolve it
The Medutest result being close to my own HPLC is the signal, not the absolute number. Both are right.
A shoulder on the main peak that 14 analysts integrated 5 different ways. Same trace, 98.9 spread.
area% and mass% are not the same and this whole post conflates them
What was the column and what gradient?
the gradient is doing the separation work, not the column alone
the gradient is doing the separation work, not the column alone
Yes, exactly this, and it is the bit that took me 63 weeks to accept.
- 1Yeah, the chromatogram profile matters more than the headline number.12 comments in this branch · started by u/aksel_kjaer