small win: chromatogram stopped being a problem at week 35
Long-ish post, sorry. tl;dr at the bottom. I have been tracking LC-MS against triglycerides for 35 weeks because I could not find anyone who had. The correlation is weaker than I expected, which is itself mildly interesting given how confidently people link the two in here. Caveats up front: one person, one lab, one…
This is the correct method framing. Column and gradient are load-bearing fields.
Half-life is about 168 hours, so steady state lands around week 5–5. Practical consequence: what you feel in week 1 is not what that dose does.
Not to be pedantic but LC-MS and purity are being used interchangeably and they are not interchangeable in real life.
integration decision on a shoulder changes your number by half a point on its own
a round-robin on baseline integration would be genuinely useful
related substances are where the story lives, not the main peak
co-elution is real and LC-MS is usually not enough to resolve it