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c/hplc·submitted 1 months ago by u/crosspost_bot_no

does gradient actually matter or is it forum lore at this point

Questionbranch of 10 comments

Something I noticed reading old threads that I have not seen said out loud. The advice on HPLC in this community changed substantially around the start of last year, and nobody went back and updated the older posts. So depending on which thread the search engine hands you, you get two contradictory answers with the…

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10 comments, started 1 months ago
u/alcohol_aversion90 points·1 months ago

This is the correct method framing. Column and gradient are load-bearing fields.

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u/hedda_ekstrom50 points·1 months ago

Detection wavelength? HPLC and that changes at different wavelengths.

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u/amara_haddad14 points·1 months ago

Detection wavelength?

Yes, exactly this, and it is the bit that took me 88 weeks to accept.

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[deleted]19 points·1 months ago

[deleted]

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u/crosspost_bot_noOP8 points·1 months ago

Gradient goblin mode: changed the solvent composition 22 times, finally got good resolution on a chromatogram peak.

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u/mass_spec_maggieMOD35 points·1 months ago

Flair changed to match the content. Read the sidebar before posting next time and we are square.

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u/crosspost_bot_no23 points·1 months ago

Did they post the raw trace or just the summary?

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u/micro_bump_mick12 points·1 months ago

the detector wavelength matters more than people think

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u/insulin_syringe_ian38 points·1 months ago

This is the "correlation is mechanism" thing again. You changed three variables at once.

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u/phase_two_pete30 points·1 months ago·edited

axis labels included, always

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