[Question] how do you actually verify HPLC
Something I noticed reading old threads that I have not seen said out loud. The advice on integration in this community changed substantially around the start of last year, and nobody went back and updated the older posts. So depending on which thread the search engine hands you, you get two contradictory answers…
I am going to push back on this slightly.
the gradient is doing the separation work, not the column alone
the gradient is doing the separation work, not the column alone
Yes, exactly this, and it is the bit that took me 71 weeks to accept.
The confident tone is doing a lot of work that the evidence is not.
integration decision on a shoulder changes your number by half a point on its own
the gradient is doing the separation work, not the column alone
Mechanistically the bit that matters is gradient. It explains most of the early profile and a decent chunk of the outcome.