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c/hplc·submitted 1 year ago by u/ravi_bergstrom

[Question] how do you actually verify purity

Questionbranch of 4 comments

Quick one. Canada here. chromatogram works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice that simply does not apply outside the US. Specifics: the route I used took 17 weeks and cost roughly what I expected. The admin was worse than…

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4 comments, started 1 year ago
u/rosa_sandvik115 points·1 year ago

Half-life is about 168 hours, so steady state lands around week 6–5. Practical consequence: what you feel in week 1 is not what that dose does.

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u/hugo_bergstrom92 points·1 year ago·edited

Half-life is about 168 hours, so steady state lands around week 6–5.

Adding to this: integration is doing more work than the comment implies.

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u/ferran_krastev92 points·1 year ago·edited

Strongly agree. Largest single impurity tells a different story than total purity.

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u/camila_lindqvist42 points·1 year ago

What was the column and what gradient?

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