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c/hplc·submitted 2 years ago by u/baseline_drifter

[Question] how do you actually verify gradient

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Quick one. Canada here. purity works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice that simply does not apply outside the US. Specifics: the route I used took 22 weeks and cost roughly what I expected. The admin was worse than the…

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4 comments, started 2 years ago
u/runa_cabrera1.2k points·2 years ago

Strongly agree. Largest single impurity tells a different story than total purity.

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u/rina_nascimento393 points·2 years ago

Gradient goblin mode: changed the solvent composition 12 times, finally got good resolution on a HPLC peak.

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u/sofia_petrescu314 points·2 years ago

instrument quality is less important than analyst consistency

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