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c/hplc·submitted 2 years ago by u/nnt_nate

integration — 21 things I got wrong before I got it right

Methodbranch of 5 comments

purity. That is the whole post, but I will justify it. Everything else people worry about in c/reconstitution is downstream of it. Titration speed, reflux, the endless dose arguments — most of it resolves if you sort purity out first, and almost nobody does. I say this having got it wrong for 9 months. My eGFR was…

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5 comments, started 2 years ago
u/sig_figs_samMOD77 points·2 years ago

Tracking number removed. It identifies both ends of a shipment.

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u/careful_gradient103 points·2 years ago

What was the column and what gradient?

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u/rina_bergstrom31 points·2 years ago

instrument quality is less important than analyst consistency

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u/reverse_image_ron13 points·2 years ago

two labs, two different baselines, two different purity numbers, both honest

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[removed]6 points·2 years ago

[removed by moderator]

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The analytical deep end: RP-HPLC methods, gradient design, integration decisions, LC-MS confirmation, amino-acid analysis, water content, and why two labs can report 98.1% and 96.4% on the same vial and both be right. Post the chromatogram, not the summary line.

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