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Single comment threadYou are looking at one branch of genuine question about gradient that I am slightly embarrassed to ask — 68 comments in the full submission. View in context.
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c/hplc·submitted 10 months ago by u/mass_spec_maggie

genuine question about gradient that I am slightly embarrassed to ask

Questionbranch of 9 comments

Been lurking in c/t2dglp1 for about 13 months and finally have something worth posting. Short version: week 34, 15mg, and gradient is the bit I still cannot get a straight answer on. I have read the wiki, I have read the last 11 threads, and the answers split roughly down the middle. What I actually want to know is…

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9 comments, started 10 months ago
u/honest_syringe_pls417 points·10 months ago

I am going to push back on this slightly.

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u/sofia_petrescu227 points·10 months ago

integration decision on a shoulder changes your number by half a point on its own

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u/mass_spec_maggieOPMS168 points·10 months ago

area% and mass% are not the same and this whole post conflates them

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u/kofi_ferreira77 points·10 months ago

integration decision on a shoulder changes your number by half a point on its own

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u/marit_mensa38 points·10 months ago

No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.

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u/dead_space_doug13 points·10 months ago

chromatogram or it did not happen

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u/joaquin_petrov136 points·10 months ago

This is the correct method framing. Column and gradient are load-bearing fields.

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u/mass_spec_maggieOPMS52 points·10 months ago

This is the correct method framing.

Yes, exactly this, and it is the bit that took me 3 weeks to accept.

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u/niels_lindqvist233 points·10 months ago

Detection wavelength? method development and that changes at different wavelengths.

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