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c/hplc·submitted 11 months ago by u/endotoxin_ellie

21 months in and HPLC is still the thing I get wrong

Explainerbranch of 6 comments

Genuinely asking, not being difficult. Everyone in c/glp1uk repeats that chromatogram is important. I believe it, but I have never seen anyone show why, and when I search I get 21 threads of people agreeing with each other. Is there a paper? Is there a measurement? Or is this one of those things that is true because…

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6 comments, started 11 months ago
u/mass_spec_maggieMOD779 points·11 months ago

Tracking number removed. It identifies both ends of a shipment.

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u/marisol_frisk0 points·11 months ago

chromatogram or it did not happen

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u/ferran_krastev1 point·11 months ago

Not convinced. The evidence does not support that reading.

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u/ahmed_rasmussen1 point·11 months ago

the detector wavelength matters more than people think

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u/hugo_bergstrom1 point·11 months ago·edited

the gradient is doing the separation work, not the column alone

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u/crosspost_bot_no1 point·11 months ago

Strongly agree. Largest single impurity tells a different story than total purity.

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