tried HPLC for 5 weeks. here is what happened.
Something I noticed reading old threads that I have not seen said out loud. The advice on chromatogram in this community changed substantially around the start of last year, and nobody went back and updated the older posts. So depending on which thread the search engine hands you, you get two contradictory answers…
Tracking number removed. It identifies both ends of a shipment.
instrument quality is less important than analyst consistency
You have restated the marketing copy. What is the actual substance here.
The related substances profile is way more interesting than the main peak %. A 98% with 24 peaks is different from a 98% that is one 25% impurity.
area% and mass% are not the same and this whole post conflates them
This is the correct method framing. Column and gradient are load-bearing fields.
co-elution is real and LC-MS is usually not enough to resolve it