stalled for 10 weeks at 5mg and I refuse to panic this time
Trying to settle this properly because the thread from last year went in circles. The claim: purity matters. The counter-claim: it is measurement error. Both sides have been asserting it confidently for about 15 months without either producing anything. What would actually settle it is 5 people measuring the same…
I am going to push back on this slightly.
integration decision on a shoulder changes your number by half a point on its own
Yeah, the chromatogram profile matters more than the headline number.
The confident tone is doing a lot of work that the evidence is not.
Not to be pedantic but purity and LC-MS are being used interchangeably and they are not interchangeable in real life.
co-elution is real and integration is usually not enough to resolve it
This is the correct method framing. Column and gradient are load-bearing fields.
area% and mass% are not the same and this whole post conflates them
a round-robin on baseline integration would be genuinely useful