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the LC-MS question that gets asked weekly, answered properly
Been lurking in c/t2dglp1 for about 6 months and finally have something worth posting. Short version: week 18, 2.5mg, and gradient is the bit I still cannot get a…
u/hydration_hank
Half your side effects are dehydration wearing a costume.
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Overview — page 2 of 5
comment on what would you tell week-1 you about LC-MS in c/hplc · 295 points · 3 months ago
Did they post the raw trace or just the summary?
comment on help me understand chromatogram, I have read the wiki twice in c/hplc · 2 points · 3 months ago
What was the column and what gradient?
comment on the HPLC thing finally clicked for me and I want to write it down in c/hplc · 13 points · 4 months ago
two labs, two different baselines, two different purity numbers, both honest
comment on [Discussion] amylin is doing more work than we give it credit for in c/cagrilintide · 7 points · 4 months ago
Yeah, amylin is doing different work than GLP-1.
comment on [Question] how do you actually verify reimbursement in c/glp1eu · 0 points · 4 months ago
Which country? Rules differ.
comment on three years of integration threads, summarised so you do not have to read them in c/hplc · 52 points · 4 months ago
Yeah, the chromatogram profile matters more than the headline number.
comment on [Discussion] can we stop arguing about EU until somebody posts a number in c/glp1eu · 1 points · 4 months ago
Strongly agree. Pricing is local.
comment on France: chromatogram, and what it actually costs here in c/hplc · 54 points · 4 months ago
area% and mass% are not the same and this whole post conflates them
comment on someone explain HPLC to me like I have not read a paper in years in c/hplc · 90 points · 5 months ago
co-elution is real and purity is usually not enough to resolve it
comment on the LC-MS question that gets asked weekly, answered properly in c/hplc · 1 points · 5 months ago
What was the column and what gradient?
comment on the LC-MS question that gets asked weekly, answered properly in c/hplc · 1 points · 5 months ago
This is the "correlation is mechanism" thing again. You changed three variables at once.
comment on the LC-MS question that gets asked weekly, answered properly in c/hplc · 1 points · 5 months ago
instrument quality is less important than analyst consistency
comment on [Meta] proposal — a flair for dose history posts in c/dosinglogs · 1 points · 5 months ago
consistency is harder than the dose change
comment on [Question] chromatogram — what am I missing here in c/hplc · 20 points · 5 months ago
Strongly agree. Largest single impurity tells a different story than total purity.
comment on [Question] chromatogram — what am I missing here in c/hplc · 12 points · 5 months ago
No. This is the kind of confident post that gets copied into a screenshot and repeated for years. Where is the evidence.
comment on the Netherlands: purity, and what it actually costs here in c/hplc · 115 points · 6 months ago
Edit to your parent would help — the concentration you quoted assumes one scenario and you have written another above it.
comment on [Discussion] reimbursement is doing more work than we give it credit for in c/glp1eu · 11 points · 6 months ago
Inter-lab variance on this kind of assay is routinely 1–2 points. Different column, different gradient, different integration.
comment on [Results] 46 weeks on 1.7mg, full numbers, ask me anything boring in c/cagrilintide · 1 points · 6 months ago
long-acting amylin is co-agonism, different satiety profile
comment on week 6 check-in — 46kg down, early fullness manageable, one thing confusing me in c/hplc · 1 points · 7 months ago
A shoulder on the main peak that 8 analysts integrated 7 different ways.
Counter-anecdote: opposite result, same dose. Which mostly tells us the variance is huge.