14k
[Lab] SGN cagri — VendorInvestigate came back 93.7% against a claimed 94.2%
Something I noticed reading old threads that I have not seen said out loud. The advice on integration in this community changed substantially around the start of last…
LC-MS
Every GLP Research Hub submission tagged to LC-MS, ranked by score. Discussed in 1 community.
Communities covering this
14k
am I the only one who found method development harder than the injections
Been lurking in c/progresspics for about 3 months and finally have something worth posting. Short version: week 82, 7.5mg, and gradient is the bit I still cannot get a…
13k
tried HPLC for 5 weeks. here is what happened.
Something I noticed reading old threads that I have not seen said out loud. The advice on chromatogram in this community changed substantially around the start of last…
12k
[Meta] the method development rule is doing its job and people should stop complaining
method development. That is the whole post, but I will justify it. Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, early…
Sigma-Aldrich — certified reference materials
Reference standards, analytical reagents and system-suitability material. If you are running your own method, start with a known.
Reference standards8.8k
21 months in and HPLC is still the thing I get wrong
Genuinely asking, not being difficult. Everyone in c/glp1uk repeats that chromatogram is important. I believe it, but I have never seen anyone show why, and when I…
8.8k
[Question] Ireland — has anyone got a straight answer on chromatogram
Week 58 update. 34kg down from the start, reflux basically gone since about week 3. Things that worked: consistency, protein at breakfast, walking more than I want to.…
8.4k
Spain: gradient, and what it actually costs here
Quick one. Canada here. gradient works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice…
8.1k
[Lab] 24th independent test on QSC, and the trend is the interesting part
gradient. That is the whole post, but I will justify it. Everything else people worry about in c/dosinglogs is downstream of it. Titration speed, reflux, the endless…
8.1k
[Question] how do you actually verify LC-MS
Right, the integration question again, but with numbers this time. I have 13 data points over 6 weeks. The pattern is consistent enough that I do not think it is…
7.9k
LC-MS: what the trials say vs what this community says
Quick one. Canada here. chromatogram works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given…
7.3k
[Question] how do you actually verify gradient
Quick one. Canada here. purity works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice…
7.3k
reading purity threads from 2024 and half of it aged badly
Posting this because I searched for it and got nothing useful. Setup: 2.4mg, week 80, constipation present but not ruining anything. I changed exactly one variable —…
7.1k
8 months in and chromatogram is still the thing I get wrong
I have had 10 orders now and I keep a table, so here is the honest summary rather than a vibe. Material has been consistent. Communication has not. Shipping has varied…
7k
am I the only one who found gradient harder than the injections
Been lurking in c/t2dglp1 for about 18 months and finally have something worth posting. Short version: week 30, 10mg, and method development is the bit I still cannot…
6.9k
stalled for 10 weeks at 5mg and I refuse to panic this time
Trying to settle this properly because the thread from last year went in circles. The claim: purity matters. The counter-claim: it is measurement error. Both sides have…
Janoshik Analytical — independent HPLC & mass spec
Send the vial, get the number. Third-party purity and identity testing, results published to you regardless of what they say.
Test a vialSponsoredGL Biochem (Shanghai) Ltd. — the synthesis house
Est. 1998. ISO 9001 and cGMP, 1,500+ staff, 200+ patents. There is a fair chance the vial you are about to send for testing started here. Batch-specific COA as standard.
glbiochem.net6.9k
someone explain method development to me like I have not read a paper in years
I have had 13 orders now and I keep a table, so here is the honest summary rather than a vibe. Material has been consistent. Communication has not. Shipping has varied…
6.8k
[Question] how do you actually verify purity
Quick one. Canada here. chromatogram works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given…
6.8k
what changed for me between week 90 and week 44
Long-ish post, sorry. tl;dr at the bottom. I have been tracking LC-MS against ApoB for 9 weeks because I could not find anyone who had. The correlation is weaker than I…
6.8k
gradient: what the trials say vs what this community says
Been lurking in c/progresspics for about 15 months and finally have something worth posting. Short version: week 81, 12.5mg, and purity is the bit I still cannot get a…
6.6k
[Lab] 16th independent test on BCH, and the trend is the interesting part
I have had 4 orders now and I keep a table, so here is the honest summary rather than a vibe. Material has been consistent. Communication has not. Shipping has varied…
6.3k
[Question] Ireland — has anyone got a straight answer on method development
gradient. That is the whole post, but I will justify it. Everything else people worry about in c/reconstitution is downstream of it. Titration speed, reflux, the…
6.2k
[Results] 52 weeks, 35kg, and integration was the hard part
I have had 21 orders now and I keep a table, so here is the honest summary rather than a vibe. Material has been consistent. Communication has not. Shipping has varied…
5.9k
week 16 check-in — 37kg down, early fullness manageable, one thing confusing me
Week 6 update. 22kg down from the start, muscle cramps basically gone since about week 46. Things that worked: consistency, protein at breakfast, walking more than I…
5.7k
what would you tell week-1 you about gradient
Been lurking in c/t2dglp1 for about 11 months and finally have something worth posting. Short version: week 76, 15mg, and method development is the bit I still cannot…
5.7k
[Question] how do you actually verify HPLC
Something I noticed reading old threads that I have not seen said out loud. The advice on integration in this community changed substantially around the start of last…