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c/reconstitution·submitted 2 years ago by u/dexa_twice_yearly

[Discussion] we are measuring mg/mL at the wrong time and calling it noise

Discussionbranch of 8 comments

Thinking out loud about this: we are measuring mg/mL at the wrong time and calling it noise. If your calculation produces a fraction of a unit, the concentration is wrong for your dose. Change the water volume until the numbers land on whole units. Research-use-only material is not approved for human use, so treat…

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8 comments, started 2 years ago
u/ilias_cabrera517 points·2 years ago

The lyophilised cake displaces a small volume, so the true concentration is very slightly lower than the arithmetic suggests. It is a fraction of a percent and it is dwarfed by fill tolerance.

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u/gradient_goblin168 points·2 years ago

Yes. Writing 5mg/mL on the vial takes four seconds and removes the entire class of error this board exists to fix.

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u/vial_math_verasyringe math57 points·2 years ago

Adding more water does not change how much peptide is in the vial. It changes the concentration, which changes the volume you draw for the same amount. That is the entire relationship.

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u/anya_antonsen39 points·2 years ago

bacteriostatic water has the preservative, sterile water does not

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u/ines_lindqvist51 points·2 years ago

Not quite. Bacteriostatic and sterile water are not interchangeable for a multi-draw vial, and the difference is the preservative.

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u/ruben_cabrera207 points·2 years ago

Yes.

gradient_goblin has the units point exactly right. They are a volume on the barrel and nothing else.

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u/escrow_evangelist0 points·2 years ago

gradient_goblin has the units point exactly right.

Right, and the arithmetic behind it: 10mg in 2mL is 5mg/mL, so 0.25mg is 5 units.

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u/emil_wojcik360 points·2 years ago

What is written on the vial right now?

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About c/reconstitution

The arithmetic and the technique. Bacteriostatic versus sterile water, reconstitution volume as a concentration choice, converting mg/mL into insulin-syringe units, dead space, swirl-not-shake, stopper coring, and how long a reconstituted vial is actually good for. Math errors get corrected fast and politely.

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