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c/reconstitution·posted 11 months ago by u/piotr_bruun

unpopular opinion: most of what gets said here about dead space is guesswork

Discussion Sourced ×5

The title is the argument: unpopular opinion: most of what gets said here about dead space is guesswork. Here is the rest of it.

The arithmetic in full: concentration is mass over volume, so 5mg into 1mL gives 5mg/mL. A U-100 syringe is graduated at 100 units per millilitre, so 0.75mg is 0.75 divided by 5 millilitres, which is 15 units.

Why "more water" keeps confusing people, including me for a month.

The vial contains a fixed amount of peptide. Water changes nothing about that amount. What it changes is concentration, and concentration is the divisor between the dose you want and the volume you draw.

So doubling the water halves the concentration and doubles the units for the same dose. Nothing is diluted in any sense that matters; you are simply reading a bigger number off the same barrel. Pick the water volume that puts your usual dose on a whole number of units and life gets easier.

If your calculation produces a fraction of a unit, the concentration is wrong for your dose. Change the water volume until the numbers land on whole units.

Tell me where this is wrong. That is the useful part of posting it.

389 up / 12 down97% upvoted28 commentsid 16vwgy18 Aug 2025

28 comments

19 in this archive, depth 3

best — the order this archive was captured in

u/saskia_mwangi0 points·11 months ago

Adding more water does not change how much peptide is in the vial. It changes the concentration, which changes the volume you draw for the same amount. That is the entire relationship.

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u/piotr_bruunOP1 point·11 months ago

Show the arithmetic — where did the unit figure come from?

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u/karim_ilunga40 points·11 months ago

Same. I got this wrong once, caught it because the units looked absurd, and have written on every vial since.

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u/piotr_bruunOP25 points·11 months ago

I keep a laminated card in the drawer with the arithmetic on it. Looks ridiculous. Has never once let me draw the wrong volume.

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u/marisol_frisk20 points·11 months ago

bacteriostatic water has the preservative, sterile water does not

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u/bac_water_banditreconstitution26 points·11 months ago·edited

The whole calculation, once, with real numbers.

You have a 10mg vial. You add 2mL of bacteriostatic water. Concentration is mass over volume: 5mg/mL. A U-100 insulin syringe is graduated at 100 units per millilitre, so one unit is 0.01mL.

To draw 0.5mg you need 0.5 divided by 5 millilitres, which is 10 units on the barrel. Write "5mg/mL" on the vial in marker before you put it away, and you never have to do this again for that vial.

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u/rina_bergstrom20 points·11 months ago

write the concentration on the vial in marker, that is the whole post

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u/sock_puppet_spotter15 points·11 months ago

Aim the stream down the wall of the vial rather than into the cake. Reconstitution is a solubility problem, not a mixing problem, and mechanical shear does nothing helpful.

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u/karl_fischer_kev12 points·11 months ago

That only works if the fill weight is exactly the label weight, and it usually is not exactly anything.

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u/ilias_steiner-16 points·11 months ago

swirl, never shake

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u/zaid_balogun1 point·11 months ago

Agreed — units are volume. Once that lands, the rest of the arithmetic is one division.

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u/kenji_kimani1 point·11 months ago·edited

Yes, and the dead-space point is worth repeating: it is small, it is real, and it is the same for every draw.

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u/piotr_bruunOP1 point·11 months ago

Correction: that gives 20 units, not what you have written. 5mg into 2mL is 2.5mg/mL and the division follows from there.

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u/anjali_tamm8 points·11 months ago

Wrote the date on the vial as well as the concentration after losing track of which one was opened first.

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u/alcohol_aversion4 points·11 months ago

Is that a U-100 syringe? The barrel markings change the answer completely.

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u/runa_cabrera4 points·11 months ago

What is written on the vial right now?

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u/reflux_report2 points·11 months ago

Not quite. Bacteriostatic and sterile water are not interchangeable for a multi-draw vial, and the difference is the preservative.

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u/units_not_mgMOD2 points·11 months ago

Corrected the concentration in the title, which contradicted the body. Nothing else touched.

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About c/reconstitution

The arithmetic and the technique. Bacteriostatic versus sterile water, reconstitution volume as a concentration choice, converting mg/mL into insulin-syringe units, dead space, swirl-not-shake, stopper coring, and how long a reconstituted vial is actually good for. Math errors get corrected fast and politely.

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