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c/reconstitution·posted 1 months ago by u/dexa_twice_yearly

how much of what we believe about mg/mL actually comes from reconstitution threads

Question The Quiet One ×8 Receipts ×1

Question in the title, detail here: how much of what we believe about mg/mL actually comes from reconstitution threads.

The arithmetic in full: concentration is mass over volume, so 10mg into 1mL gives 10mg/mL. A U-100 syringe is graduated at 100 units per millilitre, so 2.5mg is 2.5 divided by 10 millilitres, which is 25 units.

Bacteriostatic water contains benzyl alcohol as a preservative, which is what makes repeated entry into the same vial reasonable. Plain sterile water has no preservative.

The whole calculation, once, with real numbers.

You have a 20mg vial. You add 1mL of bacteriostatic water. Concentration is mass over volume: 20mg/mL. A U-100 insulin syringe is graduated at 100 units per millilitre, so one unit is 0.01mL.

To draw 5mg you need 5 divided by 20 millilitres, which is 25 units on the barrel. Write "20mg/mL" on the vial in marker before you put it away, and you never have to do this again for that vial.

Screenshot none of this. Read the whole thread, including the parts where I am told I am wrong.

1,834 up / 96 down95% upvoted27 commentsid 15sj6s28 Jun 2026

27 comments

11 in this archive, depth 4

best — the order this archive was captured in

u/cardio_endpoint_c218 points·1 months ago

Why "more water" keeps confusing people, including me for a month.

The vial contains a fixed amount of peptide. Water changes nothing about that amount. What it changes is concentration, and concentration is the divisor between the dose you want and the volume you draw.

So doubling the water halves the concentration and doubles the units for the same dose. Nothing is diluted in any sense that matters; you are simply reading a bigger number off the same barrel. Pick the water volume that puts your usual dose on a whole number of units and life gets easier.

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u/honest_syringe_maybe54 points·1 months ago

the number you write down is the number you will trust at 6am

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[removed]78 points·1 months ago

[removed by moderator]

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u/hedda_ekstrom176 points·1 months ago

Correction: that gives 40 units, not what you have written. 5mg into 2mL is 2.5mg/mL and the division follows from there.

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u/arto_falk174 points·1 months ago·edited

Spent 9 weeks confused about this until somebody drew the table for me. 20mg, 2mL, 10mg/mL, 1mg is 10 units. That is the whole thing.

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u/kavya_radich77 points·1 months ago

bacteriostatic water has the preservative, sterile water does not

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u/RetaAndRegret235 points·1 months ago·edited

aim the stream at the glass wall, not at the cake

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u/elin_ferrari19 points·1 months ago

Yes, and the dead-space point is worth repeating: it is small, it is real, and it is the same for every draw.

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u/alcohol_aversion0 points·1 months ago

Yes, and the dead-space point is worth repeating: it is small, it is real, and it is the same for every draw.

Right, and the arithmetic behind it: 20mg in 2mL is 10mg/mL, so 1.5mg is 15 units.

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u/dexa_twice_yearlybody comp63 points·29 days ago

The lyophilised cake displaces a small volume, so the true concentration is very slightly lower than the arithmetic suggests. It is a fraction of a percent and it is dwarfed by fill tolerance.

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u/ines_ekstrom-18 points·1 months ago·edited

5mg into 2mL is 2.5mg/mL, and 0.25mg of that is 10 units

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About c/reconstitution

The arithmetic and the technique. Bacteriostatic versus sterile water, reconstitution volume as a concentration choice, converting mg/mL into insulin-syringe units, dead space, swirl-not-shake, stopper coring, and how long a reconstituted vial is actually good for. Math errors get corrected fast and politely.

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