reading non-peptide threads from 2024 and half of it aged badly
reading non-peptide threads from 2024 and half of it aged badly. Making the case below, and I expect to lose some of it in the comments. The no-timing-restriction thing is the part I would care about most in practice, and it barely gets mentioned here. Assumed the peptide purity conversations transferred and they do…
The analytical point, which this board keeps getting wrong by importing habits from the peptide side.
For a peptide, purity is typically reported as area percent by HPLC with identity by mass spectrometry, and the impurity classes are things like deletion and oxidation products. For a small molecule the relevant impurities are synthetic intermediates, degradants and residual solvents, and identity is established differently.
So the certificate you would want looks different, the questions to ask are different, and a "purity" figure quoted here is not comparable to one quoted on the peptide boards. Anybody posting a result should say what method produced it, which is good practice everywhere and essential here.
Compared milligram figures across two completely different molecule classes in a comment. Deserved the correction.
Corrected a cross-class dose comparison in the title. The body is untouched.
Daily dosing, so what does the exposure profile look like across the day?
Spent an evening reading about small-molecule agonism at a peptide receptor. Genuinely interesting engineering.
identity testing on a small molecule is a different analytical problem