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c/hplc·submitted 11 days ago by u/micro_bump_mick

bought a second-hand HPLC. worst best decision of my life. AMA

Labbranch of 6 comments

Quick one. Canada here. HPLC works very differently where I live than in the threads that dominate the front page, and I keep seeing people confidently given advice that simply does not apply outside the US. Specifics: the route I used took 2 weeks and cost roughly what I expected. The admin was worse than the cost.…

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6 comments, started 9 days ago
u/dead_space_doug18 points·9 days ago

Detection wavelength? method development and that changes at different wavelengths.

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u/quiet_reader_990 points·9 days ago

The Janoshik result being close to my own HPLC is the signal, not the absolute number. Both are right.

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u/rasmus_petrescu1 point·9 days ago

area% and mass% are not the same and this whole post conflates them

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The analytical deep end: RP-HPLC methods, gradient design, integration decisions, LC-MS confirmation, amino-acid analysis, water content, and why two labs can report 98.1% and 96.4% on the same vial and both be right. Post the chromatogram, not the summary line.

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