help me understand LC-MS, I have read the wiki twice
The title is the whole question — help me understand LC-MS, I have read the wiki twice — but here is why I am asking. Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient. Change any of those and the number changes without the sample changing. Reading a…
Gave the same trace to two people I trust and got two integrations about a point apart. That was the most educational afternoon I have had here.
Yes — system suitability first. Without it the number is an assertion about the instrument, not the sample.
That is area percent, not mass percent. The trace cannot give you the second one.
Chased a ghost peak for three weeks. It was the plumbing. It is always the plumbing.
A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves.
A shoulder that does not baseline-resolve cannot be quantified honestly.
Disagreeing with this bit: that spread is ordinary inter-lab variance, not a disagreement about the material.
That figure cannot be quoted to two decimals off that baseline. The precision is not in the data.
Ran a blank after a high-concentration injection and found the carryover I had been calling an impurity.