[PSA] chromatogram is not what most of this community thinks it is
chromatogram is not what most of this community thinks it is. Two minutes of reading now saves an argument later. Started reporting my own integrations with the baseline choice stated. Arguments in my threads dropped by about half. Reading a trace posted here, in the order I actually look at things. Axes first — if…
A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves.
two labs, two gradients, two honest answers
reproducibility beats resolution if you only get one of them
That is area percent, not mass percent. The trace cannot give you the second one.
a shoulder is not an impurity until you can resolve it
Same method at both labs, or two different gradients?
report the method or do not report the number