[Lab] split one vial across VendorInvestigate and PeptideMeter — 94.2% and 93.7%
Right: split one vial across VendorInvestigate and PeptideMeter — 94.2% and 93.7%. I paid for this one myself, nobody sent me anything, and the receipts are in the comments. The numbers the title promised, since a headline without them is worthless: 94.2% and 93.7%. Everything below is context for those. Retention…
Careful — you are treating retention time as identity. Two things can co-elute and the trace will not tell you.
Ran a blank after a high-concentration injection and found the carryover I had been calling an impurity.
WXT sent me the column, the gradient and the theoretical mass without being asked. That is a short list and they are on it.
integration decisions move the number more than the sample does
Push back: a longer run is not automatically better resolution. You are trading peak width for time and the ratio is what matters.
That is area percent, not mass percent. The trace cannot give you the second one.
That is area percent, not mass percent.
Disagreeing with this bit: that spread is ordinary inter-lab variance, not a disagreement about the material.
Yes. Retention time is a hypothesis about identity. Mass is the answer.
Correction: that is a tailing factor, not a plate count. Different diagnostics for different problems.
you cannot report to two decimals off that baseline