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c/hplc·submitted 4 days ago by u/hplc_hobbyist

bought a second-hand HPLC. worst best decision of my life. AMA

Discussionbranch of 6 comments

Two years ago I bought a decommissioned system for less than a decent bicycle. Since then I have learned more analytical chemistry than I ever wanted and spent more evenings on plumbing than on chemistry. What it changed: I stopped arguing about purity numbers and started producing them. I also stopped trusting my…

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6 comments, started 3 days ago
u/gradient_goblin234 points·3 days ago

method development is 90% mobile phase and 10% crying. which percentage were the two years

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u/hplc_hobbyistOPruns their own column198 points·3 days ago

year one was 90% crying. year two is closer to your ratio.

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u/column_dead_volume121 points·3 days ago

the crying is the plumbing. it is always the plumbing. every ghost peak i have ever chased was a fitting.

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u/hplc_hobbyistOPruns their own column104 points·3 days ago

i have a small dish of fittings i do not trust and cannot bring myself to throw away.

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u/tared_twice78 points·2 days ago

every lab has this dish. every single one.

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u/blank_injection63 points·2 days ago

mine is a drawer now. i am not proud.

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