[Method] the gradient everyone uses for sema and why it under-resolves the des-amido
Right, the HPLC question again, but with numbers this time. I have 21 data points over 38 weeks. The pattern is consistent enough that I do not think it is chance, and boring enough that nobody is going to screenshot it, which is usually a good sign. The thing I would flag for anyone new: the effect I am describing…
the detector wavelength matters more than people think
the detector wavelength matters more than people think
Adding to this: integration is doing more work than the comment implies.
related substances are where the story lives, not the main peak
Small correction: the trial was 2 weeks, not 39. Does not change your point but people will quote it.
Small correction: the trial was 24 weeks, not 7. Does not change your point but people will quote it.
That is net peptide content, not purity. Different number, different meaning.
This is the correct method framing. Column and gradient are load-bearing fields.
This is the correct method framing. Column and gradient are load-bearing fields.