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c/hplc·submitted 5 months ago by u/valeria_cardoso

reading HPLC threads from 2024 and half of it aged badly

Methodbranch of 10 comments

reading HPLC threads from 2024 and half of it aged badly. I have gone back and forth on this for months. System suitability — repeat injections, tailing factor, plate count, RSD on area — is what tells you the instrument was fit for the measurement that day. Without it, the purity figure is unanchored. Area percent…

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10 comments, started 4 months ago
u/ewan_marchand78 points·4 months ago

a shoulder is not an impurity until you can resolve it

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u/georgi_tamm-23 points·4 months ago

ghost peak, check the plumbing first, it is always the plumbing

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u/ewan_zielinski1 point·4 months ago

Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.

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u/nora_lundgren1 point·4 months ago

ghost peak, check the plumbing first, it is always the plumbing

georgi_tamm is right — the integration choice is a decision and it should be stated alongside the result.

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