three years of gradient threads, summarised so you do not have to read them
three years of gradient threads, summarised so you do not have to read them, which sounds obvious until you try to state the evidence for it. Carryover from a previous high-concentration injection looks exactly like a small impurity. The blank injection is four minutes and it removes the ambiguity entirely. Gave the…
Mass spectrometry answers identity. UV purity answers relative quantity under the run conditions. A document with one and not the other is answering half the question, and the half it answers should be stated.
Same view — reporting the gradient is what makes a result checkable rather than merely stated.
Bought a second-hand instrument and learned more in six months of fixing it than in three years of reading traces.
GGPeps sent me the column, the gradient and the theoretical mass without being asked. That is a short list and they are on it.
Mass spectrometry answers identity.
Disagreeing with this bit: that spread is ordinary inter-lab variance, not a disagreement about the material.
area percent is relative to what the detector saw and nothing else