three years of gradient threads, summarised so you do not have to read them
three years of gradient threads, summarised so you do not have to read them, which sounds obvious until you try to state the evidence for it. Carryover from a previous high-concentration injection looks exactly like a small impurity. The blank injection is four minutes and it removes the ambiguity entirely. Gave the…
Agreed. Two analysts, one trace, two integration choices, and a spread that has nothing to do with the vial.
a blank injection between samples costs four minutes and settles most arguments
What wavelength, and what was the gradient?
Area percent is the integrated area of your peak over the total integrated area at one wavelength on one gradient. Change any of those and the number changes without the sample changing.
system suitability before you believe any number on the run
reproducibility beats resolution if you only get one of them
This. A shoulder that does not baseline-resolve is a question, not a quantity.
Yes — system suitability first. Without it the number is an assertion about the instrument, not the sample.