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c/hplc·submitted 1 year ago by u/aksel_kjaer

[Question] is 99.2% actually fine or am I being sold a rounding error

Questionbranch of 7 comments

Title says it — is 99.2% actually fine or am I being sold a rounding error — so here is the boring supporting detail that makes it worth anything. The relevant figures are 99.2%, and they come from the same log I have kept the whole time. The system suitability argument, since it comes up whenever somebody posts a…

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7 comments, started 1 year ago
u/first_hundred18 points·1 year ago

Yes. Retention time is a hypothesis about identity. Mass is the answer.

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u/hplc_hobbyistruns their own column11 points·1 year ago·edited

What wavelength, and what was the gradient?

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u/hazard_ratio_halstats6 points·1 year ago

That is area percent, not mass percent. The trace cannot give you the second one.

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u/ignacio_vanhecke5 points·1 year ago

Push back: a longer run is not automatically better resolution. You are trading peak width for time and the ratio is what matters.

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u/ravi_bergstrom5 points·1 year ago

System suitability — repeat injections, tailing factor, plate count, RSD on area — is what tells you the instrument was fit for the measurement that day. Without it, the purity figure is unanchored.

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u/kofi_ferreira4 points·1 year ago

Gave the same trace to two people I trust and got two integrations about a point apart. That was the most educational afternoon I have had here.

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u/quiet_reader_991 point·1 year ago

Ran a blank after a high-concentration injection and found the carryover I had been calling an impurity.

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