[Discussion] chromatogram is doing more work than we give it credit for
chromatogram is doing more work than we give it credit for — a position I have arrived at slowly and would like tested. A shoulder that does not baseline-resolve cannot be quantified honestly. You can report it as an unresolved shoulder, which is useful information, or you can develop the method until it resolves.…
Resolution between two peaks depends on retention, selectivity and efficiency. A shallower gradient buys retention and usually resolution, at the cost of peak width and run time.
Resolution between two peaks depends on retention, selectivity and efficiency.
Agreed. And the corollary is that the method line on a certificate is not decoration, it is the number’s provenance.
Small fix — 214nm, not 210. It matters for the comparison you are making with the other run.
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Sent the same vial to Medutest and VendorInvestigate. 97.6% against a claimed 97.5%. The difference was the gradient, not the material.
area percent is relative to what the detector saw and nothing else
Gave the same trace to two people I trust and got two integrations about a point apart. That was the most educational afternoon I have had here.
integration decisions move the number more than the sample does