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c/coldchain·posted 2 years ago by u/ms_fragmenter

[Meta] the lyophilised rule is doing its job and people should stop complaining

Data Receipts ×4 Sourced ×2 Cold Box ×2

the lyophilised rule is doing its job and people should stop complaining. Nothing about this affects the ranking maths, before anyone asks.

Moved everything off the fridge door after reading a thread here and my temperature log immediately got boring.

Gel pack fully liquid on arrival, powder tested 97.6% against a claimed 97.5% afterwards. The pack had done its job for as long as it was designed to.

Put a logger in three consecutive orders. Two never went above the range at all and the third spent four hours warm on a Friday. Cost me almost nothing to stop wondering.

Research-use-only material is not approved for human use and nothing here should be read as a recommendation to use it.

3,453 up / 374 down90% upvoted51 commentsid v49ydw18 Dec 2023

51 comments

30 in this archive, depth 5

best — the order this archive was captured in

u/kian_ekstrom301 points·2 years ago

Once in solution, the relevant risks are hydrolysis, aggregation and, for some sequences, oxidation. Refrigeration slows all three; freezing a solution introduces a separate risk at the ice interface.

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u/vito_chowdhury208 points·2 years ago

Gel packs are designed for a duty cycle. A liquid pack at the end of a longer-than-expected transit is a pack behaving as specified, not evidence of an excursion in the vial.

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u/ms_fragmenterOP94 points·2 years ago

Ordered from the warehouse in Germany instead of origin and the transit went from a fortnight to 7 days. Entirely different proposition in August.

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u/usb_logger_ursulaMOD117 points·2 years ago

Merged into the seasonal thread for that lane so the reports sit together.

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u/bence_ibarra51 points·2 years ago

That is the ambient temperature at the destination, not the temperature inside the box. The logger measures the second one.

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[removed]24 points·2 years ago

[removed by moderator]

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u/sequence_checkerresearch peptides63 points·2 years ago

ask which lines are held in regional stock before you order in July

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u/tomas_lokken113 points·2 years ago

Lyophilisation removes the water that drives most degradation pathways. That is why the dry cake is comparatively robust and the reconstituted solution is not.

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u/honest_syringe33-12 points·2 years ago

A transit temperature logger records a distribution, and time above a threshold matters as much as peak temperature. That is the argument for logging rather than guessing.

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u/rohan_steiner1 point·2 years ago

if you want to know, log it, otherwise you are guessing

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u/ms_fragmenterOP1 point·2 years ago

A transit temperature logger records a distribution, and time above a threshold matters as much as peak temperature.

Adding the practical version: ask which lines are held regionally before you order in July.

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u/ms_fragmenterOP1 point·2 years ago

powder at room temperature for a few days is usually a non-event

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u/analog_alphabet1 point·2 years ago

Small fix — refrigerated, not frozen, once it is in solution.

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u/kian_solberg1 point·2 years ago

Which lane, and had it been quoted at that transit time?

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u/nayeli_iyer1 point·2 years ago

ask what storage condition the stability statement was written against

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u/sequence_checkerresearch peptides92 points·2 years ago

Regional stock changes the question rather than answering it: shorter lanes mean less exposure and fewer variables, which is why the warehouse question is worth asking before ordering in summer.

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u/soren_yildiz42 points·2 years ago

insulation buys hours, not days

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u/milan_mensah75 points·2 years ago

Disagree with the panic framing. Lyophilised material tolerates ordinary transit conditions far better than these threads imply.

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u/emil_marchand74 points·2 years ago·edited

A stability statement is only meaningful with its conditions attached: temperature, duration, light exposure, and whether it covers powder or solution. Ask which.

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u/isabela_nilsen71 points·2 years ago

the excursion that matters is the one after reconstitution

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u/milan_mensah20 points·2 years ago

a warm gel pack tells you the pack failed, not that the material did

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u/cesar_teixeira32 points·2 years ago

a logger costs less than a vial and answers the question permanently

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u/sten_palacios22 points·2 years ago

write the reconstitution date on the vial

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u/spain_receta30 points·2 years ago

lyophilised powder is far more forgiving than the panic threads suggest

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Temperature and stability in the real world: what an excursion actually costs you, why lyophilised powder is far more forgiving than reconstituted solution, how to read a logger CSV, and how to photograph a failed shipment so the conversation with the vendor is short.

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