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c/coa·submitted 3 months ago by u/priya_palacios

[Lab] 18th independent test on HJ — 99.2% on a claimed 99.0%, and the trend is the interesting part

Labbranch of 13 comments

18th independent test on HJ — 99.2% on a claimed 99.0%, and the trend is the interesting part. One vial, one service, one member paying, which is the only kind of result this board should treat as evidence. 99.2% and 99.0%. Those are measured, not estimated, and not rounded up in my favour. My first certificate had a…

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13 comments, started 3 months ago
u/tomas_lehtinen87 points·3 months ago

Net peptide content is the fraction of the powder that is actually peptide once counterion, water and residual solvent are accounted for. A 99% pure acetate salt can still be about 85% peptide by mass.

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u/amylin_amyamylin67 points·3 months ago

The PDF creation date being later than the analysis date is usually a re-issue or a re-export. It is worth one question and it is not evidence of anything on its own.

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u/dario_boateng97 points·3 months ago

Water content 3.8%, acetate 6.2%, purity 99%, net peptide about 90% of label weight. All four numbers honest, and I had to read it twice.

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u/priya_palaciosOP50 points·3 months ago·edited

Does the lot on the vial match the lot on the page?

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u/camila_lindqvist39 points·3 months ago

Pushing back a little: a missing field is usually presentation, not concealment. Ask for the full document before drawing conclusions.

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[removed]26 points·3 months ago

[removed by moderator]

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u/coa_janitorMOD34 points·3 months ago·edited

Removed — cropped document. Post the whole page or do not post it; the fields people crop are the fields that matter.

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u/laila_wojcik26 points·3 months ago

HPLC-UV and LC-MS answer different questions

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u/sofia_danquah20 points·3 months ago·edited

post the whole document, not the headline number

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u/priya_palaciosOP14 points·3 months ago

Correction: that is net peptide content, not purity. Different number, different meaning, and the document says so if you read the row label.

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u/sofia_ferreira38 points·3 months ago

net peptide content and purity are two different numbers on the same vial

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u/step_therapy_s12 points·3 months ago

Are the related substances itemised or is it a single total?

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u/anecdata_annie44 points·3 months ago

chromatogram or it did not happen

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How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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