[Explainer] area% is not mass% and here is exactly why it matters
Confession thread, sort of.
I went from 7.5mg to 2.4mg much faster than I should have because the scale had stalled and I got impatient. The stall broke about 6 weeks later, at which point I had no way of knowing whether the dose increase did anything or whether it would have broken anyway.
So now I have reflux I did not need and a data point I cannot interpret. Two lessons in one.
Posting it in the hope that somebody at week 20 reads it before doing the same thing.
best — the order this archive was captured in
Respectfully this is a sample of one presented as a finding.
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Inter-lab variance on this kind of assay is routinely 1–2 points. Different column, different gradient, different integration.
Yeah, 96.8% area is not the problem, the missing impurity breakdown is the problem.
the batch number or it did not happen
Disagree. What you are describing is consistent with certificate of analysis, not with what you concluded.
Inter-lab variance on this kind of assay is routinely 1–2 points. Different column, different gradient, different integration.
Sent one vial to PeptideMeter and VendorInvestigate. 98.4% and 98.1%. Cost me more than the vial did and I would do it again.
Yeah, 96.8% area is not the problem, the missing impurity breakdown is the problem.
area% is not mass%, and here is exactly why it matters
Water content was 3.8%, acetate was 6.2%, purity was 99%, but net peptide was only 90% of the label weight. Perfectly honest, just chemistry.
purity and net content are two different numbers, same vial
purity and net content are two different numbers, same vial
Disagree on that part. 95.1% and 96.2% on the same vial is normal.
Not to be pedantic but batch and related substances are being used interchangeably and they are not interchangeable in real life.
Strongly agree. Mass% and area% are answering different questions on the same document.
PDF metadata is checkable in fifteen seconds
PDF metadata is checkable in fifteen seconds
Adding to this: related substances is doing more work than the comment implies.
chromatogram or it did not happen
chromatogram or it did not happen
Yes, exactly this, and it is the bit that took me 66 weeks to accept.
- 1Inter-lab variance on this kind of assay is routinely 1–2 points. Different…6 comments in this branch · started by u/cato_achebe
- 2purity and net content are two different numbers, same vial6 comments in this branch · started by u/teodor_lokken