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c/coa·submitted 1 year ago by u/clara_danquah

unpopular opinion: most of what gets said here about certificate of analysis is guesswork

Labbranch of 12 comments

unpopular opinion: most of what gets said here about certificate of analysis is guesswork — a position I have arrived at slowly and would like tested. LC-MS gives you identity; UV purity gives you a relative quantity. A document with the first and not the second, or the reverse, is answering half the question. Asked…

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12 comments, started 1 year ago
u/anders_kuusela-14 points·1 year ago

Ask what the stability statement was written against — temperature, light, and whether it was the lyophilised powder or the reconstituted solution. Those are three different claims.

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[removed]1 point·1 year ago

[removed by moderator]

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u/dario_vermeulen1 point·1 year ago

That is the catalogue certificate rather than the batch-specific one. Same layout, completely different status.

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u/clara_danquahOP1 point·1 year ago

axis labels included, always

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u/rohan_steiner1 point·1 year ago

chromatogram or it did not happen

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u/valeria_karlsen1 point·1 year ago

net peptide content and purity are two different numbers on the same vial

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u/clara_danquahOP1 point·1 year ago

ask for the storage condition the stability statement was written against

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u/sofia_danquah1 point·1 year ago

Did you get the chromatogram or only the summary line?

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u/ahmed_rasmussen1 point·1 year ago

The PDF creation date being later than the analysis date is usually a re-issue or a re-export. It is worth one question and it is not evidence of anything on its own.

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u/ahmed_rasmussen1 point·1 year ago

area% is not mass%, and the difference is the whole game

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u/clara_danquahOP1 point·1 year ago

Is water content on the document? It changes your concentration arithmetic.

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u/tidy_vialdrawer_watch281 point·1 year ago

the related substances line is where the story is

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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