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c/coa·posted 1 year ago by u/tidy_vialdrawer_watch28

help me understand batch, I have read the wiki twice

Lab Receipts ×3 Long Haul ×1 Clean Column ×1

Question in the title, detail here: help me understand batch, I have read the wiki twice.

The PDF creation date being later than the analysis date is usually a re-issue or a re-export. It is worth one question and it is not evidence of anything on its own.

Sent one vial to VendorInvestigate and PeptideMeter. 98.8% and a claimed 98.0%. Cost me more than the vial and I would do it again.

Started keeping a folder of every certificate I have been sent. The ones that itemise impurities are a small group and they are mostly the same suppliers.

If somebody has the same thing measured a different way, post it next to mine and we will see whether they agree.

10,648 up / 1,476 down88% upvoted43 commentsid 1yxl6328 Oct 2024

43 comments

11 in this archive, depth 3

best — the order this archive was captured in

u/sig_figs_sammod · analytical1k points·1 year ago·edited

Net peptide content is the fraction of the powder that is actually peptide once counterion, water and residual solvent are accounted for. A 99% pure acetate salt can still be about 85% peptide by mass.

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u/teodor_jansen-20 points·1 year ago

Ask what the stability statement was written against — temperature, light, and whether it was the lyophilised powder or the reconstituted solution. Those are three different claims.

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u/anya_correia705 points·1 year ago

Is water content on the document? It changes your concentration arithmetic.

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u/milos_vanhecke376 points·1 year ago

Did you get the chromatogram or only the summary line?

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u/milos_trevino173 points·1 year ago

Did you get the chromatogram or only the summary line?

milos_vanhecke has the distinction right — one is a fraction of the detector signal, the other is a fraction of the mass.

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u/india_generic_g206 points·1 year ago

This. Area percent answers "what fraction of what the detector saw was the peak I wanted", and people read it as "how much peptide is in the vial".

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u/nikhil_lindqvist445 points·1 year ago

axis labels included, always

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u/laila_wojcik347 points·1 year ago

Spent four months confusing area% and mass% in every post I made here. One explainer thread and it clicked permanently.

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u/coa_janitorMOD191 points·1 year ago

Reminder: a question about a document is not an accusation about a supplier, and posts that blur the two get retitled.

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About c/coa

How to read a certificate of analysis and what it does and does not tell you. Area% versus mass%, identity versus quantity, water content, related substances, acetate counterions, and the difference between a vendor-supplied COA and an independent test on the vial you actually received.

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c/coa rules
  1. Post the full document. Cropped COAs that hide the batch number or date get removed.
  2. Redact your own name and order id, not the vendor's batch details.
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